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Vourekas, A. et al. Mili and Miwi target RNA repertoire reveals piRNA biogenesis and function of Miwi in spermiogenesis. Nat. Struct. Mol. Biol. 19, 773–781 (2012). del Campo, E. M. Post-transcriptional control of chloroplast gene expression. Gene Regul. Syst. Bio. 3, 31–47 (2009). Niranjanakumari, S., Lasda, E., Brazas, R. & Garcia-Blanco, M. A. Reversible cross-linking combined with immunoprecipitation to study RNA–protein interactions in vivo. Methods 26, 182–190 (2002).

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Max, K. E. A. et al. Human plasma and serum extracellular small RNA reference profiles and their clinical utility. Proc. Natl Acad. Sci. USA 115, E5334–E5343 (2018).

Bailey, T. L. et al. MEME SUITE: tools for motif discovery and searching. Nucleic Acids Res. 37, W202–W208 (2009). Livi, C. M., Klus, P., Delli Ponti, R. & Tartaglia, G. G. catRAPID signature: identification of ribonucleoproteins and RNA-binding regions. Bioinformatics 32, 773–775 (2016). A process in which an intron is spliced sequentially in two or more distinct steps. Biomolecular condensates So let's continue with the same data set from the last example.If you refer to the image from the last example, you'll see that the text is overflowing into the cells to the right of column B. Which of the following options will make the text in the cell extend beyond the cell limits when the cell(s) to the right are blank?

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Formicola, N., Vijayakumar, J. & Besse, F. Neuronal ribonucleoprotein granules: dynamic sensors of localized signals. Traffic 20, 639–649 (2019). Branon, T. C. et al. Efficient proximity labeling in living cells and organisms with TurboID. Nat. Biotechnol. 36, 880–887 (2018). More than 100 RNA modifications have been described; most affect the assembly of protein–RNA complexes and therefore should be integrated into studies of protein–RNA interactions. Interestingly, mutations of certain methyltransferases can stabilize covalently linked protein–RNA catalytic intermediates, thus enabling CLIP to be performed without the need for UV cross-linking, as has been done for m5C-miCLIP 207. Most methods to date have been developed for transcriptomic studies of m 6A, the type of modification that is most common in mRNAs, and these include variants of CLIP, such as m 6A-miCLIP, which employ antibodies that recognize m 6A-containing RNA 208. The success of such approaches critically depends on the quality of the antibodies recognizing the modification 209. Therefore, similar to studies of protein–RNA interactions, integration of data from complementary methods will be valuable to gain a full picture of RNA modifications and their roles in RNP assembly 202, 210. Gottwein, E. et al. Viral microRNA targetome of KSHV-infected primary effusion lymphoma cell lines. Cell Host Microbe 10, 515–526 (2011).

Wheeler, D. L. et al. Database resources of the national center for biotechnology information. Nucleic Acids Res. 36, D13–D21 (2008). Uren, P. J. et al. High-throughput analyses of hnRNP H1 dissects its multi-functional aspect. RNA Biol. 13, 400–411 (2016).